SINGLE-CELL MULTIOMICS
OUR MULTIOMICS FACILITIES
C-TRIC’s state-of-the-art multiomics facilities enhances clinical research by providing researchers access to High-Throughput Single-Cell Multiomics and Next Generation Sequencing (NGS) services and training.
These technologies are essential for understanding complex molecular changes contributing to normal development, cellular response, and understanding disease.
C-TRIC is a My Green Lab Ambassador and is passionate about promoting sustainability within our labs.
Pre PCR
Sample QC
Single Cell Isolation
Post PCR
Library QC
Sequencing
Single-Cell Multiomics Services
High Throughput Single-Cell Multiomics
A state-of-the-art high-throughput, microwell-based single-cell partitioning system, combined with unique visual workflow QC, enables multiomic analysis of single cells.
It allows the discovery of new biomarkers of disease, novel cell types and states, and the identification of potential candidates for new therapeutics.
The range of applications includes:
- Single-cell RNA-seq (gene expression)
- Single-cell protein expression (intracellular and extracellular)
- Immune profiling (T-cell/B-cell receptor analysis)
- Single-cell CRISPR screening
- Single-cell ATAC-seq (methylation)
State-of-the-art high-throughput, microwell-based Single-Cell partitioning system, combined with visual workflow QC, enabling co-detection of gene and protein expression within single cells.
Allows the discovery of new biomarkers of disease, novel cell types and states and the identification of potential candidates for new therapeutics.
The range of applications includes:
- Single-cell RNA-seq (Gene expression)
- Single-cell protein expression
- T-cell receptor/B-cell receptor analysis
- Single Cell CRISPR Screening
BD Rhapsody Single-Cell Scanner and High Throughput Xpress Systems
Why use single-cell multiomics?
Single-Cell Analysis vs. Traditional Analysis
(Fruit Salad vs. Smoothie)
Simultaneously analysing gene, protein, and methylation expression from single cells is crucial for understanding cell biology, disease mechanisms, and immune responses.
SINGLE-CELL RESOLUTION
Insights into the variation between individual cells within a sample (fruit salad) rather than the average from a population of different cells (smoothie). This is essential for understanding disease at a cellular level, allowing precise and more effective therapeutics to be developed.
Insights into the variation between individual cells within a sample (fruit salad) rather than the average from a population of different cells (smoothie). This is essential for understanding disease at a cellular level, allowing precise and more effective therapeutics to be developed.
Multiomic Profiling
Simultaneously analysing gene and protein expression from Single Cells. It is crucial for understanding cell biology, including cell differentiation, disease mechanisms, and immune responses.
Simultaneously analysing gene and protein expression from Single Cells. It is crucial for understanding cell biology, including cell differentiation, disease mechanisms, and immune responses.
SCALABLE
Scale experiments to match specific needs, from small-scale projects to high-throughput studies, all can benefit cost-effective research planning.
Scale experiments to match specific needs, from small-scale projects to high-throughput studies, all can benefit cost-effective research planning.
APPLICATIONS
Adaptable to various sample types, including tissues and cell suspensions, it is suitable for various research applications within precision medicine, e.g., immunology, neuroscience, blood cancer, cardiometabolic disease, etc.
Adaptable to various sample types, including tissues and cell suspensions, it is suitable for various research applications within precision medicine, e.g., immunology, neuroscience, blood cancer, cardiometabolic disease, etc.
BD Rhapsody Key Selling Points
- Million cell studies now possible!
- Process up to 192 samples (24 per lane) in ~ 2 hours over eight lanes with up to 440,000 cells per cartridge
- Real-time decisions on single cell QC including cell viability, cell capture and multiplet rate
- Save time and sequencing costs by troubleshooting single-cell capture
- Gentle microwell technology ideal for fragile cells
- No sample loss due to clogging of channels
- Recover cells with varied size and morphology
- 2.5% @ 10,000 cell load
- BD® OMICS-Guard Sample Preservation Buffer protects multiomic information for up to 72 hours at 4 °C
- Stable cDNA capture allows transfer to multiple research sites for downstream analysis without requirement for cold shipping
- Archive cell capture beads with equivalent data obtained from fresh beads
- Vital backup for low quality library preps
Other Multiomic Services
Flow Cytometry
- Immunophenotyping
- Cell sorting
- Cell viability
Next Generation Sequencing (NGS)
- NGS Quality control (fragment analysis, etc.)
- Genomics (e.g., Targeted sequencing, microbial WGS etc.)
- Transcriptomics (e.g., RNA-seq, 16s rRNA, etc.)
- Whole exome sequencing
Bioinformatic Analysis
- Primary
- Secondary
Sample Requirements
- We will assess the quantity and quality of the DNA/RNA you submit upon receipt using sensitive/specific techniques available (Qubit for concentration measurements, fragment analysis for size distribution and checking for degradation) and inform you of the results before proceeding. For libraries prepared yourselves (‘ready-prepared libraries’), see below.
- Please contact us if you would like to discuss using any protocol other than those listed above. For any queries please don’t hesitate to contact the team.
- Sample requirements listed below are recommendations from kit suppliers however for samples that fall below this we can advise of next steps.
- For 1-24 samples please have samples in single tubes or plate format.
- For 25-96 samples please have samples in plate format.
- Download PDF
- We can sequence Illumina libraries you have prepared yourself. Please pool and prepare an aliquot appropriate as specified in the table below.
Instruments
A state-of-the-art high-throughput, microwell-based single-cell partitioning system, combined with unique visual workflow QC, enables multiomic analysis of single cells.
It allows the discovery of new biomarkers of disease, novel cell types and states, and the identification of potential candidates for new therapeutics.
The range of applications includes:
- Single-cell RNA-seq (gene expression)
- Single-cell protein expression (intracellular and extracellular)
- Immune profiling (T-cell/B-cell receptor analysis)
- Single-cell ATAC-seq (methylation)
- Single-cell CRISPR screening
Illumina’s NextSeq 2000 is a cutting-edge next generation sequencing (NGS) system.
Applications range from targeted sequencing to data-rich Multiomics applications including:
- Single Cell Multiomics
- Genomics (Targeted sequencing + metagenomics)
- Transcriptomics (RNA-seq + 16s rRNA sequencing)
- Whole exome sequencing
Achieves up to 3.6 billion paired-end reads and reads of up to 600bp in length.
High-performance flow cytometry with superior multicolour performance.
Fluidics and optical systems are precisely integrated to maximize signal detection, allowing the system to achieve high sensitivity and resolution.
Applications include:
- Immunophenotyping
- Isolation of Single Cell populations
- Cell viability
Ancillary Equipment
High throughput fragment analysis system for: RNA/DNA quality control, NGS library quality control and PCR fragment sizing.
Dual-mode microplate reader (absorbance/ fluorescence) for high throughput, low volume nucleic acid quantification.
Rapid, accurate polymerase chain reaction (PCR) in combination with real-time, online detection, enabling absolute or relative quantification of a target nucleic acid, as well as post-PCR analysis of the amplified nucleic acid by melting curve analysis.
Highly accurate and precise automated low-volume pipetting with built-in UV lamp and HEPA filters to prevent contamination.
High Throughput Isothermal Amplification System based on the LAMP (Loop-mediated isothermal amplification) technology, which allows for the rapid amplification of nucleic acid sequences under isothermal conditions that enables the detection of even low-level nucleic acid targets.
JOIN OUR MISSION
Shape the future of healthcare by participating in clinical research. We are always in need of new recruits and your involvement could make a difference for both yourself and future generations.
We are especially keen to hear from individuals with cardiovascular and heart disease.
