SINGLE-CELL MULTIOMICS

OUR MULTIOMICS FACILITIES

C-TRIC’s state-of-the-art multiomics facilities enhance clinical research by providing access to High-Throughput Single-Cell Multiomics, Next Generation Sequencing (NGS) services and training.

These technologies are essential for understanding complex changes in normal development, cellular response, and understanding disease processes to allow development of more effective treatments. We can identify novel targets of disease, improve biomarker identification and monitor disease progression / drug response. Our mission is to help improve patient care.

C-TRIC is a My Green Lab Ambassador and is passionate about promoting sustainability within our labs.

C-TRIC offers bespoke project design, access to latest NGS and single-cell multiomics services, high quality nucleic acid extractions/ quality control and competency training in NGS/ multiomics.  

Specific multiomic services highlighted below, please contact us to discuss your specific requirements and explore how we can help you to achieve your project goals.

Pre PCR

RNA/DNA extractions
Sample QC
Single Cell Isolation

Post PCR

Library Prep
Library QC
Sequencing

Single-Cell Multiomics Services

High Throughput Single-Cell Multiomics

A state-of-the-art high-throughput, microwell-based single-cell partitioning system, combined with unique visual workflow QC, enables multiomic analysis of single cells.

It allows the discovery of new biomarkers of disease, novel cell types and states, and the identification of potential candidates for new therapeutics.

The range of applications includes:

  • Single-cell RNA-seq (gene expression)
  • Single-cell protein expression (intracellular and extracellular)
  • Immune profiling (T-cell/B-cell receptor analysis)
  • Single-cell CRISPR screening
  • Single-cell ATAC-seq (methylation)

State-of-the-art high-throughput, microwell-based Single-Cell partitioning system, combined with visual workflow QC, enabling co-detection of gene and protein expression within single cells.

Allows the discovery of new biomarkers of disease, novel cell types and states and the identification of potential candidates for new therapeutics.

The range of applications includes:

  • Single-cell RNA-seq (Gene expression)
  • Single-cell protein expression
  • T-cell receptor/B-cell receptor analysis
  • Single Cell CRISPR Screening

BD Rhapsody Single-Cell Scanner and High Throughput Xpress Systems

Why use single-cell multiomics?

Single-Cell Analysis vs. Traditional Analysis
(Fruit Salad vs. Smoothie)


SINGLE-CELL RESOLUTION

Insights into the variation between individual cells within a sample (fruit salad) rather than the average from a population of different cells (smoothie). This is essential for understanding disease at a cellular level, allowing precise and more effective therapeutics to be developed.

Insights into the variation between individual cells within a sample (fruit salad) rather than the average from a population of different cells (smoothie). This is essential for understanding disease at a cellular level, allowing precise and more effective therapeutics to be developed.

Multiomic Profiling

Simultaneously analysing gene and protein expression from Single Cells. It is crucial for understanding cell biology, including cell differentiation, disease mechanisms, and immune responses.

Simultaneously analysing gene, protein expression and methylation from Single Cells. It is crucial to take a multiomics approach to better understanding cell biology, including cell differentiation, disease mechanisms, and immune responses.

SCALABLE

Scale experiments to match specific needs, from small-scale projects to high-throughput studies, all can benefit cost-effective research planning.

Scale experiments to match specific needs, from small-scale projects to high-throughput studies, all can benefit from cost-effective research planning.

APPLICATIONS

Adaptable to various sample types, including tissues and cell suspensions, it is suitable for various research applications within precision medicine, e.g., immunology, neuroscience, blood cancer, cardiometabolic disease, etc.

Investigation of disease mechanisms to identify potential biomarkers and therapeutic targets. Evaluate the impact of therapeutic compounds on cells/ tissues of interest aiding in the development of precision treatments.  

Adaptable to various sample types, including tissues and cell suspensions, it is suitable for various research applications within precision medicine, e.g., immunology, neuroscience, blood cancer, cardiometabolic disease, etc. 

BD Rhapsody Key Selling Points

High Throughput
  • Million cell studies now possible!
  • Process up to 192 samples (24 per lane) in ~ 2 hours over eight lanes with up to 440,000 cells per cartridge
Visual Workflow QC
  • Real-time decisions on single cell QC including cell viability, cell capture and multiplet rate
  • Save time and sequencing costs by troubleshooting single-cell capture

    Rhapsody visual workflow graphic

Maintain Sample Integrity
  • Gentle microwell technology ideal for fragile cells
  • No sample loss due to clogging of channels
  • Recover cells with varied size and morphology
Low Multiplet Rate
  • 2.5% @ 10,000 cell load
Experimental Flexibility
  • BD® OMICS-Guard Sample Preservation Buffer protects multiomic information for up to 72 hours at 4 °C
  • Stable cDNA capture allows transfer to multiple research sites for downstream analysis without requirement for cold shipping
  • Archive cell capture beads with equivalent data obtained from fresh beads
  • Vital backup for low quality library preps

Other Multiomic Services

Flow Cytometry

  • Immunophenotyping
  • Cell sorting
  • Cell viability

Next Generation Sequencing (NGS)

  • Genomics (e.g., Targeted sequencing, microbial WGS etc.)
  • Transcriptomics (e.g., RNA-seq, 16s rRNA, etc.)
  • Whole exome sequencing
  • NGS Quality control (Fragment analysis, nucleic acid quantification etc.)

Bioinformatic Analysis

  • Primary
  • Secondary

Sample Requirements

General Submission Advise
  • We will assess the quantity and quality of the DNA/RNA you submit upon receipt using sensitive/specific techniques available (Qubit for concentration measurements, fragment analysis for size distribution and checking for degradation) and inform you of the results before proceeding. For libraries prepared yourselves (‘ready-prepared libraries’), see below.
  • Please contact us if you would like to discuss using any protocol other than those listed above. For any queries please don’t hesitate to contact the team.
  • Sample requirements listed below are recommendations from kit suppliers however for samples that fall below this we can advise of next steps.
  • For 1-24 samples please have samples in single tubes or plate format.
  • For 25-96 samples please have samples in plate format.
  • Download PDF

     

Ready-Prepared Libraries for Illumina Sequencing
  • We can sequence Illumina libraries you have prepared yourself. Please pool and prepare an aliquot appropriate as specified in the table below.

Instruments

BD Rhapsody Single Cell Scanner and High Throughput Xpress Systems

A state-of-the-art high-throughput, microwell-based single-cell partitioning system, combined with unique visual workflow QC, enables multiomic analysis of single cells.

It allows the discovery of new biomarkers of disease, novel cell types and states, and the identification of potential candidates for new therapeutics.

The range of applications includes:

  • Single-cell RNA-seq (gene expression)
  • Single-cell protein expression (intracellular and extracellular)
  • Immune profiling (T-cell/B-cell receptor analysis)
  • Single-cell ATAC-seq (methylation)
  • Single-cell CRISPR screening
 

Illumina NextSeq 2000 

Illumina’s NextSeq 2000 is a cutting-edge next generation sequencing (NGS) system.

Applications range from targeted sequencing to data-rich Multiomics applications including:

  • Single Cell Multiomics
  • Genomics (Targeted sequencing + metagenomics)
  • Transcriptomics (RNA-seq + 16s rRNA sequencing)
  • Whole exome sequencing

Achieves up to 3.6 billion paired-end reads and reads of up to 600bp in length.

BD FACSAria III – Flow Cytometer

High-performance flow cytometry with superior multicolour performance.

Fluidics and optical systems are precisely integrated to maximize signal detection, allowing the system to achieve high sensitivity and resolution.

Applications include:

  • Immunophenotyping
  • Isolation of Single Cell populations
  • Cell viability

Ancillary Equipment

Agilent Fragment Analyzer 5300
  • High throughput fragment analysis system for: RNA/DNA quality control, NGS library quality control and PCR fragment sizing.

Tecan Infinite Nano +

Dual-mode microplate reader (absorbance/ fluorescence) for high throughput, low volume nucleic acid quantification.

Roche LightCycler 480 II Real-Time PCR System

Rapid, accurate polymerase chain reaction (PCR) in combination with real-time, online detection, enabling absolute or relative quantification of a target nucleic acid, as well as post-PCR analysis of the amplified nucleic acid by melting curve analysis.

EzMate 601s Automated Pipetting System

Highly accurate and precise automated low-volume pipetting with built-in UV lamp and HEPA filters to prevent contamination.

Genie HT Isothermal Amplification System

High Throughput Isothermal Amplification System based on the LAMP (Loop-mediated isothermal amplification) technology, which allows for the rapid amplification of nucleic acid sequences under isothermal conditions that enables the detection of even low-level nucleic acid targets.

JOIN OUR MISSION

Shape the future of healthcare by participating in clinical research. We are always in need of new recruits and your involvement could make a difference for both yourself and future generations.

We are especially keen to hear from individuals with cardiovascular and heart disease.


Please let us know if you have any of the following conditions: